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Allergen‐induced recruitment of FcσRI + eosinophils in human atopic skin
Author(s) -
Barata Luis T.,
Ying Sun,
Andrew Grant J.,
Humbert Marc,
Barkans Julia,
Meng Qui,
Durham Stephen R.,
Barry Kay A.
Publication year - 1997
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830270527
Subject(s) - allergen , eosinophil , polyclonal antibodies , immunoglobulin e , immunology , biology , microbiology and biotechnology , receptor , allergy , monoclonal antibody , in situ hybridization , in vivo , antibody , messenger rna , biochemistry , asthma , gene
Abstract We have attempted to identify FcσRI + eosinophils in cutaneous late‐phase reaction in atopic subjects biopsied at 6, 24 and 48 h after the injection of either allergen or a diluent control. Compared to the diluent sites, allergen‐injected sites had significantly increased numbers of eosinophils, peaking between 6 and 24 h, of which approximately 20–30% expressed mRNA for the α, β, and γ chains of FcσRI, as shown by in situ hybridization. Using either a monoclonal or a polyclonal anti‐α chain antibody, the FcσRIα protein also co‐localized to approximately 50–80% of eosinophils at all time points studied. We also observed a significant correlation (r = 0.89; p = 0.02) between the numbers of FcσRI + (997 + )/EG2 + eosinophils and the magnitude of the late‐phase reaction. Thus, a significant proportion of eosinophils infiltrating the site of allergen‐induced allergic tissue reactions in atopic subjects express FcσRI. The findings show that high‐affinity IgE receptors may play a role in eosinophil secretory processes in vivo .