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Interleukin‐10 and interleukin‐4 have similar effects on hapten‐specific primary antibody responses to penicillin in vivo
Author(s) -
Kerdine Saadia,
Pallardy Marc,
Lamanètre Sandrine,
Bertoglio Jacques,
Lebrec Hervé
Publication year - 1996
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830261213
Subject(s) - biology , neutralization , in vivo , toxoid , immunology , cytokine , antibody , microbiology and biotechnology , hapten , isotype , immune system , immunization , monoclonal antibody
Abstract Interleukin (IL)‐10 was initially recognized on the basis of its capacity to inhibit production of interferon (IFN)‐γ by T helper (Th)1 lymphocytes; we examined whether this cytokine can bias the primary antibody (Ab) response to the hapten penicillin. We previously reported that BALB/c and SJL mice develop different responses to benzylpenicillin coupled to tetanus toxoid (BPO‐TT). The response of SJL mice was characterized as Th2 on the basis of early and high IL‐4 mRNA expression and production of BPO‐specific Ab of the IgG1 isotype. In contrast, the response of BALB/c mice was characterized as Th1 on the basis of delayed and weaker IL‐4 mRNA expression associated with high anti‐BPO IgG2a production (Kerdine, S. et al., Mol. Immunol . 1996. 33 : 71). In this report, we demonstrate that in naive animals, the level of expression of IL‐10 mRNA in LN cells was high in SJL and barely detectable in BALB/c. In addition, injection of BPO‐TT resulted in rapid and large increase of IL‐10 mRNA expression in SJL. Neutralization of IL‐10 in vivo promoted the production of BPO‐specific IgG2a in SJL, and injection of IL‐10‐CHO cells inhibited BPO‐specific IgG2a production in BALB/c. Neutralization on administration of IL‐10 had effects very similar to neutralization or administration of IL‐4. However, co‐neutralization of IL‐10 and IL‐4 in SJL did not evidence additive or synergistic effects of the two cytokines. Administration of IL‐10 or IL‐4 in BALB/c inhibited BPO‐TT‐induced expression of IL‐12 p40 mRNA without modulating IFN‐γ mRNA. Together, these data demonstrate that endogenous production of IL‐10 regulates the production of IgG2a Ab in response to BPO‐TT and that IL‐10, like IL‐4, is critical for controlling primary responses to antibiotics which behave as haptenic compounds.