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Cleavage of membrane‐bound CD40 ligand is not required for inducing B cell proliferation and differentiation
Author(s) -
Pietravalle Fabienne,
LecoanetHenchoz Sybille,
Aubry JeanPierre,
Elson Greg,
Bonnefoy JeanYves,
Gauchat JeanFrançcois
Publication year - 1996
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830260333
Subject(s) - biology , cleavage (geology) , microbiology and biotechnology , cd40 , membrane , biochemistry , in vitro , cytotoxic t cell , fracture (geology) , paleontology
Abstract The phycical interaction between the B cell surface molecule CD40 and its ligand, CD40L, is known to be crucial in the development and maintenance of humoral immunity. Recently it has been shown that the CD40L is processed and that its soluble cleavage products are released into the extracellular environment. To study the functions of soluble and membrane‐bound human CD40L on human B cells, we generated an uncleavable CD40L cDNA deletion mutant. The activities of transfectants expressing either mutated or wild‐type CD40L were then compared on human B cells. Both the soluble and the uncleavable membrane‐bound CD40L were able to induce, in conjunction with interleukin‐4, B cell proliferation and IgE synthesis. Therefore, membrane‐bound and soluble CD40L exhibit the same pattern of activities on B cells and membrane CD40L cleavage is not a prerequisite for its function.