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A study of complexes of class II invariant chain peptide: Major histocompatibility complex class II molecules using a new complex‐specific monoclonal antibody
Author(s) -
Eastman Susan,
Deftos Michael,
Deroos Paul C.,
Hsu DiHwei,
Teyton Luc,
Braunstein Ned S.,
Hackett Charles J.,
Rudensky Alexander
Publication year - 1996
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830260218
Subject(s) - cd74 , major histocompatibility complex , biology , mhc class ii , mhc restriction , antigen presentation , monoclonal antibody , microbiology and biotechnology , mhc class i , mutant , antigen , peptide , antigen processing , endosome , antibody , t cell , biochemistry , gene , immune system , immunology , intracellular
Abstract Complexes of major histocompatibility complex (MHC) class II molecules containing invariant chain (Ii)‐derived peptides, known as class II‐associated invariant chain peptides (CLIP), are expressed at high levels in presentation‐deficient mutant cells. Expression of these complexes in mutant and wild‐type antigen‐presenting cells suggests that they represent an essential intermediate in the MHC class II antigen‐presenting pathway. We have generated a monoclonal antibody, 30‐2, which is specific for these complexes. Using this antibody, we have found quantitative differences in CLIP: MHC class II surface expression in mutant and wild‐type cells. Our experiments also show that CLIP: MHC class II complexes are preferentially expressed on the cell surface similar to total mature MHC class II molecules. These complexes are found to accumulate in the endosomal compartment in the process of endosomal Ii degradation. Analysis of the fine specificity of the antibody indicates that these complexes have Ii peptide bound to the peptide‐binding groove.