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Identification of an ionomycin/cyclosporin A‐responsive element within the human T cell receptor gamma enhancer
Author(s) -
Hettmann Thore,
Cohen Amos
Publication year - 1995
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830251223
Subject(s) - ionomycin , enhancer , biology , transcription factor , microbiology and biotechnology , calcineurin , receptor , gene , intracellular , genetics , medicine , transplantation
Abstract Activation through the Ca 2+ /calcineurin pathway is essential to the transcription of many cytokine genes. The conserved cis ‐acting sequence, GGAAAA, and transcription factors binding to this sequence are involved in the response to increased intracellular Ca 2+ concentrations. Here we report the identification and importance of the same sequence in a non‐cytokine gene, the human T cell receptor gamma (TCRG) enhancer. Results from site‐directed mutations and electrophoretic mobility shift assays strongly suggest that this sequence mediates the ionomycin‐induced activation of the TCRG enhancer. Our studies provide an explanation for a previous observation that TCRG mRNA levels, but not mRNA levels for T cell receptor alpha and ‐beta, are increased by ionomycin treatment.