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Reconstitution of a functional interleukin (IL)‐7 receptor demonstrates that the IL‐2 receptor γ chain is required for IL‐7 signal transduction
Author(s) -
Ziegler Steven F.,
Morella Karen K.,
Anderson Dirk,
Kumaki Noriko,
Leonard Warren J.,
Cosman David,
Baumann Heinz
Publication year - 1995
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830250214
Subject(s) - biology , signal transduction , receptor , microbiology and biotechnology , interleukin 1 receptor , common gamma chain , interleukin 1 receptor, type i , interleukin 15 , interleukin 4 , interleukin , interleukin 21 receptor , immunology , cytokine , genetics
Abstract The interleukin (IL)‐2 receptor γ chain has recently been shown to be a component of the IL‐7 and IL‐4 receptors. Using a transient transfection assay and the trans ‐activation of reporter gene constructs which are under the control of cytokine‐responsive promoter elements, we have studied signal transduction through the IL‐7 receptor (IL‐7R). The reporter gene expression was not stimulated by receptors that contained the cytoplasmic domain of the IL‐7R, either as intact IL‐7R or as part of a chimeric receptor. However, co‐expression of the IL‐7R with the IL‐2 receptor γ chain was able to stimulate gene activation. For maximal stimulation the intact cytoplasmic domains of each chain was required.