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Distinct structural and functional epitopes of the α E β 7 integrin
Author(s) -
Russell Gary J.,
Parker Christina M.,
Cepek Karyn L.,
Mandelbrot Didier A.,
Sood Arvind,
Mizoguchi Emiko,
Ebert Ellen C.,
Brenner Michael B.,
Bhan Atul K.
Publication year - 1994
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830241138
Subject(s) - epitope , monoclonal antibody , biology , integrin , microbiology and biotechnology , intraepithelial lymphocyte , alpha (finance) , antibody , antigen , gel electrophoresis , immunology , biochemistry , cell , immune system , medicine , construct validity , nursing , patient satisfaction
Abstract Intestinal intraepithelial lymphocytes (iIEL) are predominantly CD3 + , CD8 + T lymphocytes located above or adjacent to the mucosal basement membrane. Although they are positioned to interact with intercellular luminal antigen or with enterocytes, the function of iIEL remains unknown. Most (> 85%) of the iIEL express the α E β 7 integrin which appears to be involved in the adhesion of lymphocytes to epithelial cells. We report the characterization of three monoclonal antibodies (mAb) termed αE7‐1, αE7‐2, and αE7‐3, that react with the α E β 7 integrin recognized by the previously described mAb HML‐1 as demonstrated by identical sodium dodecyl sulfate – polyacrylamide gel electrophoresis mobility and charge. Flow cytometric analysis of antibody cross‐blocking indicated that these mAb recognize distinct epitopes of α E β 7 . While all of the mAb were capable of blocking the adhesion of cultured iIEL to a breast epithelial cell line, only HML‐1 and αE7‐1 (which recognize an identical or closely related epitope) were co‐stimulatory with suboptimal concentrations of anti‐CD3 mAb in inducing proliferation of cultured iIEL. Thus, these mAb appear to recognize functionally distinct epitopes of α E β 7 and will be useful to study relationships between the structure and function of this integrin.