Premium
Involvement of p21 ras activation in T cell CD69 expression
Author(s) -
d'Ambrosio Daniele,
Cantrell Doreen A.,
Frati Luigi,
Santoni Angela,
Testi Roberto
Publication year - 1994
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830240319
Subject(s) - jurkat cells , biology , t cell receptor , microbiology and biotechnology , t cell , guanosine , cd3 , reporter gene , transfection , gene expression , antigen , immune system , biochemistry , gene , immunology , cd8
Abstract The involvement of p21 ras in the induction of the early activation antigen CD69 was investigated in T cells. Expression of a v‐Ha‐ ras coding for a constitutively active ras protein in Jurkat cells resulted in CD69 induction on the cell surface. Transfected ras was shown to be constitutively activated and functionally efficient, since it could be immunoprecipitated in the guanosine triphosphate (GTP)‐bound form and it induced transactivation of an AP‐1 consensus‐chloramphenicol acetyltransferase reporter gene. The requirement for ras activation in T cell receptor (TcR) CD3‐mediated CD69 induction was also investigated. The expression of a dominant negative c‐Ha‐ ras ‐N17 mutant markedly reduced the amount of GTP that could be immunoprecipitated from ras proteins after TcR/CD3 triggering in Jurkat cells, and concomitantly decreased TcR/CD3‐mediated CD69 induction. These results suggest a central role for ras in TcR/CD3‐mediated CD69 expression in T cells.