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Switching capacity of FcϵRII‐positive and ‐negative murine B cells
Author(s) -
Foy Teresa M.,
Waldschmidt Thomas J.
Publication year - 1993
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830231225
Subject(s) - immunoglobulin e , biology , cd23 , spleen , marginal zone , immunology , b cell , isotype , receptor , allotype , antibody , lipopolysaccharide , immunoglobulin d , microbiology and biotechnology , monoclonal antibody , biochemistry
Abstract In previous studies, our laboratory demonstrated the utility of the low affinity IgE Fc receptor (FcϵRII) in delineating a number of murine B cell subsets. In the spleen, the FcϵRII is expressed on mature conventional B cells but is absent on marginal zone B cells. In the peritoneal cavity, the receptor is present on all conventional B cells, but is not expressed on fresh peritoneal Ly1/sister B cells. The studies in this report compared the ability of these B cell poputations to isotype switch. Using a lipopolysaccharide (LPS)‐ and interleukin (IL)‐4‐driven system, sort‐purified FcϵRII‐ positive and ‐negative B cells from peritoneum and spleen were tested for switching to IgG1, IgE, and IgA. The results demonstrated that regardless of their source, FcϵRII + B cells produced significant levels of IgG1 and IgE. Similar results were obtained with FcϵRII − (marginal zone) B cells obtained from spleen. In contrast, FcϵRII − (Ly1/sister) peritoneal B cells were found to produce IgG1 and IgA, but were incapable of secreting significant levels of IgE. Further studies tested for LPS and IL‐4‐induced expression of FcϵRII and Thy1 on the various B cell populations. These experiments demonstrated the induction of the FcϵRII on all B cells, regardless of their initial resting levels. Additionally, Thy1 was found to be induced only on those B cell subsets capable of producing IgE. Taken together, the results demonstrate a correlation between IgE secretion and Thy1 expression, and no apparent correlation between the presence of the FcϵRII and isotype commitment.