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Signaling of programmed cell death induction in WEHI‐231 B lymphoma cells
Author(s) -
Hibner Urszula,
Benhamou Laure E.,
Haury Matthias,
Cazenave PierreAndré,
Sarthou Pierre
Publication year - 1993
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830231115
Subject(s) - biology , programmed cell death , lymphoma , apoptosis , context (archaeology) , antibody , microbiology and biotechnology , cell culture , signal transduction , gene , b cell , cancer research , immunology , genetics , paleontology
Abstract The murine WEHI‐231 B lymphoma is highly sensitive to membrane immunoglobulin ligation which leads to programmed cell death (PCD) in this cell line. To study the molecular pathways involved in PCD induction in these cells, we derived two variants of WEHI‐231 resistant to anti‐Ig treatment. The level of bcl‐2 mRNA was identical in the wild type and the variants, either untreated or anti‐Ig treated, suggesting that PCD is not under the control of bcl‐2 in WEHI‐231 cells. In contrast, c‐myc gene expression was markedly different in the wild type and the variants, both in the unstimulated and anti‐Ig‐stimulated state. Our findings are interpreted in the context of the dual capacity of c‐myc to promote cell growth or cell death, in conjunction with other growth regulatory signals.