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CD21 expressed on basophilic cells is involved in histamine release triggered by CD23 and anti‐CD21 antibodies
Author(s) -
Bacon Kevin,
Gauchat JeanFrancois,
Aubry JeanPierre,
Pochon Sibylle,
Graber Pierre,
Henchoz Sybille,
Bonnefoy JeanYves
Publication year - 1993
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830231054
Subject(s) - cd23 , monoclonal antibody , histamine , microbiology and biotechnology , biology , immunoglobulin e , basophilic , antibody , chemistry , immunology , endocrinology , pathology , medicine
Abstract Recombinant full‐length human CD23 incorporated into fluorescent liposomes was used to detect a ligand for CD23 on the basophilic leukemia cell line, KU 812. Based on our recent finding that CD23 interacts with CD21 on subsets of B and T cells, we investigated if the same ligand was involved on KU 812 cells. An anti‐CD21 monoclonal antibody (mAb) BU‐33, was able to totally block CD23‐liposome binding to KU 812 cells. Moreover, KU 812 cells express CD21 mRNA and have a cell surface molecule that reacts with anti‐CD21 mAb. The CD23/CD21 interaction was not merely physical but was also associated with an increase in histamine release by KU 812 cells. Both recombinant soluble CD23 and an anti‐CD21 mAb increased histamine release by KU 812 cells. The CD23 and anti‐CD21 mAb‐mediated effect on histamine release was not restricted to the leukemic cell line, but was also observed with normal human blood basophils. These data demonstrate that CD21 is expressed on basophilic cells and that CD21 controls histamine production upon ligand‐induced stimulation (CD23 or anti‐CD21 mAb).