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Rapid transformant selection by human complement using HRF20 (CD59) cDNA as a selection marker
Author(s) -
Takizawa Hisao,
Takahashi Kazuhiro,
Murakami Tomoko,
Okada Noriko,
Okada Hidechika
Publication year - 1993
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830231052
Subject(s) - biology , cd59 , complementary dna , heterologous , transfection , complement system , microbiology and biotechnology , complement membrane attack complex , complement (music) , incubation , selection (genetic algorithm) , genetics , gene , phenotype , biochemistry , antibody , complementation , artificial intelligence , computer science
Abstract The 20 kDa homologous restriction factor (HRF20, CD59) prevents formation of membrane attack complexes of human complement. Transfection of the cDNA of HRF20 to heterologous cells, which are sensitive to human complement, thereby renders these cells resistant to human complement attack. This property can then be used as a selection marker of transfection. In addition, the procedure is short and can be performed within 8 h. Since only 1 h is required for incubation with complement, the damage to the transfected cells is limited compared with damage resulting from selection for drug resistance which requires 10 days or more of incubation in the presence of harmful agents.