Premium
Molecular characterization of the early activation antigen CD69: A type II membrane glycoprotein related to a family of natural killer cell activation antigens
Author(s) -
Ziegler Steven F.,
Ramsdell Fred,
Hjerrild Kathryn A.,
Armitage Richard J.,
Grabstein Kenneth H.,
Hennen Kathryn B.,
Farrah Terry,
Fanslow William C.,
Shevach Ethan M.,
Alderson Mark R.
Publication year - 1993
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830230737
Subject(s) - biology , cd69 , microbiology and biotechnology , complementary dna , clone (java method) , t cell , antigen , natural killer cell , phorbol , gene , cytotoxic t cell , biochemistry , il 2 receptor , immune system , signal transduction , protein kinase c , in vitro , immunology
Abstract CD69 is a disulfide‐linked homo‐dimer expressed on the surface of activated T cells, B cells, natural killer cells, neutrophils and platelets. Antibody cross‐linking of CD69 in the presence of phorbol ester results in cellular activation events including proliferation and the induction of specific genes. Using an expression cloning strategy we have isolated cDNA encoding human CD69 from a CD4 + T cell clone. Transfection of the cDNA clone in CV‐1/EBNA cells results in the expression of a covalently linked homodimer. The cDNA insert hybridizes to a 1.7‐kb mRNA in phorbol 12‐myristate 13‐acetate‐ or phytohemoagglutinin‐stimulated human T cells. Using the human clone we have isolated cDNA encoding mouse CD69, which, when expressed in human T cells allowed those cells to respond to anti‐mouse CD69 antibodies by secreting interleukin‐2 and interferon‐γ. Sequence analysis showed that both mouse and human CD69 are type II membrane glycoproteins related to the NKR‐P1 and Ly‐49 families of natural killer cell activation molecules.