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Expression of interleukin (IL)‐1β, IL‐6 and their respective receptors in the normal rat brain and after injury
Author(s) -
Yan Hong Qu,
Banos Miguel Alcaros,
Herregodts Patrick,
Hooghe Robert,
HooghePeters Elisabeth L.
Publication year - 1992
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830221131
Subject(s) - in situ hybridization , biology , receptor , immunocytochemistry , interleukin , central nervous system , extracellular , messenger rna , lesion , pathology , microbiology and biotechnology , cytokine , immunology , endocrinology , gene , biochemistry , medicine
Abstract The expression of interleukin (IL)‐1β, IL‐6 and their respective receptors has been studied in the rat brain before and up to 24 h after injury. Messenger RNA transcripts of these four genes were detected by in situ hybridization (ISH) in different structures of the intact brain. The distribution was very similar for IL‐1β, IL‐6 and IL‐6 receptor (IL‐6R). The expression of IL‐1R was more widespread. Within hours after injury, an increased expression of IL‐1β, and thereafter of IL‐6 was documented. The expression of IL‐1R and IL‐6R was also increased. This expression was bilateral and not restricted to the injured area. Within 24 h, all ISH patterns had returned to normal. The molecular data were confirmed by protein data. Indeed, the distribution of IL‐6 (detected by immunocytochemistry) agreed with the ISH patterns for IL‐6. Furthermore, extracellular fluid was collected by microdialysis at the site of the lesion during 12 h and successive fractions were assayed for the presence of bioactive IL‐1 and IL‐6. Increases in IL‐1 and later in IL‐6 levels were detected. The rapid and concomitant increased expression of IL‐1β, IL‐6 and their receptors after injury stresses their possible early role in inflammatory mechanisms also in the brain, before any recruitment of inflammatory cells from remote nervous and not nervous areas.