Premium
CD3 + T cells in severe combined immunodeficiency (scid) mice. IV. Graft‐ vs . ‐host resistance of H‐2 d scid mice to intravenous injection of allogeneic H‐2 b (C57BL/6) spleen cells
Author(s) -
Claesson Mogens H.,
Rudolphi Angelika,
Tscherning Thomas,
Reimann Jörg
Publication year - 1991
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830210913
Subject(s) - severe combined immunodeficiency , spleen , cd8 , immunology , cytotoxic t cell , adoptive cell transfer , intraperitoneal injection , cd3 , biology , immunodeficiency , transplantation , in vitro , t cell , microbiology and biotechnology , in vivo , medicine , immune system , pharmacology , biochemistry
Abstract Intraperitoneal injection of 2 × 10 7 nonfractionated spleen cells (SC) from C57BL/6 (B6, H‐2 b ) mice into completely allogeneic immunodeficient H‐2 d scid mice induced clinical and histological signs of acute graft‐ vs. ‐host disease (GVHD), with all transplanted severe combined immunodeficiency (scid) mice dying in the 3rd week post‐transfer. In contrast four out of five scid mice survived for > 7 weeks after intravenous (i.v.) injections of equal numbers of B6 SC. Intravenously allotransplanted scid mice analyzed in the 8th week post‐transfer had engrafted donor‐type CD4 + and CD8 + T cells in the spleens but showed no clinical or histological evidence of GVHD. i.v. injection of 10 7 or 10 6O B6 SC engrafted allogeneic T cells in spleens of scid recipients; in contrast, i.v. injection of 10 5 nonfractionated B6 SC or 3 × 10 5 cell sorter‐purified, naive or anti‐H‐2 d ‐primed splenic CD4 + or CD8 + B6 T cells led to rejection by young scid recipient mice. B6 T cells engrafted into spleens of scid mice after i.v. injection showed proliferative anti‐host alloreactivity in vitro. No cytotoxic reactivity against host‐type alloantigens was found in standard 4‐h 51 Cr‐release assays. These data demonstrate that allogeneic T cells injected i.v. into immunodeficient scid mice are partially tolerized against host‐type alloantigens.