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Interleukins 1, 4 and 6 induce the colony formation of human bone marrow B lineage cells
Author(s) -
McGinnes Kimberley,
Paige Christopher J.
Publication year - 1991
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830210526
Subject(s) - biology , bone marrow , lineage (genetic) , secretion , immunology , antibody , cytokine , microbiology and biotechnology , endocrinology , biochemistry , gene
Abstract An agar‐based, B cell colony assay (McGinnes, K. et al., Blood 1990, 76 : 896) has been used to study the influence of known cytokines on the growth of B lineage colonies initiated by cells from normal, human bone marrow samples. We demonstrate that a combination of interleukin (IL) 1, IL 4 and IL 6 act directly to promote the generation of plaque‐forming colonies. IL 6 was shown to act at a late stage of colony formation, which is consistent with its role in the induction of immunoglobulin secretion from mature B cells. In contrast, IL 1 and IL 4 were required at earlier stages in the formation of colonies containing cells which secrete immunoglobulin.