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A unique subset of normal murine CD4 + T cells lacking Thy‐1 is expanded in a murine retrovirus‐induced immunodeficiency syndrome, MAIDS
Author(s) -
Holmes Kevin L.,
Morse Herbert C.,
Makino Masahiko,
Hardy Richard R.,
Hayakawad Kyoko
Publication year - 1990
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830201237
Subject(s) - spleen , biology , murine leukemia virus , retrovirus , virology , population , virus , lymph node , lymph , feline immunodeficiency virus , immunology , immunodeficiency syndrome , t cell , flow cytometry , antibody , mink , immunodeficiency , microbiology and biotechnology , immune system , lentivirus , pathology , viral disease , medicine , ecology , environmental health
Abstract Some strains of mice inoculated with LP‐BM5 murine leukemia virus (MuLV) develop a syndrome, termed mouse acquired immunodeficiency syndrome (MAIDS), characterized by progressive lymphoproliferation and profound immunodeficiency. LP‐BM5 MuLV is a virus mixture that contains ecotropic (eco) and mink cell focus‐induced MuLV and a defective genome that is the proximal cause of disease. Flow cytometry analyses of spleen and lymph nodes from susceptible C57BL/6 mice infected with this virus mixture revealed the presence in spleen and peripheral lymph nodes of a previously unrecognized subset of CD4 + CD3 + T cells that are Thy‐1 − . The frequency of these cells increased with progression of disease, eventually comprising between 30% and 50% of all CD4 + cells. Infection of A/J mice, a strain which is genetically resistant to development of MAIDS, did not induce an increase of this T cell population, indicating that infection with the virus mixture was insufficient to induce its proliferation. A central role for the defective virus in this process was suggested by the finding that C57BL/6 mice infected with LP‐BM5 eco alone did not have increased frequencies of Thy‐1 − CD4 + cells in spleen. Studies of spleen and peripheral lymph node cells from normal mice demonstrated the presence of Thy‐1 − CD4 + cells at frequencies of 1%–2%. Studies using two anti‐T cell monoclonal antibodies, SM6C10 and SM3G11, that define four CD4 + subsets showed that Thy‐1 − CD4 + T cells from normal and infected mice were present only in the 6C10 − subsets.