Premium
Recognition of a particular HLA‐DQ heterodimer by a human γ/δ T cell clone
Author(s) -
Bosnes Vidar,
Qvigstad Erik,
Lundin Knut E. A.,
Thorsby Erik
Publication year - 1990
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830200704
Subject(s) - t cell receptor , biology , cd8 , cytotoxic t cell , microbiology and biotechnology , monoclonal antibody , cd3 , peripheral blood mononuclear cell , clone (java method) , t cell , t lymphocyte , human leukocyte antigen , antigen , gene , antibody , immunology , genetics , in vitro , immune system
Abstract Peripheral blood mononuclear cells from a single donor were depleted of T cell receptor (TcR) α/β T cells, stimulated with allogeneic cells, and γ/δ T lymphocyte clones (TLC) were isolated by limiting dilution. Five TLC were cytotoxic against B‐lymphoblastoid cell lines from the stimulating cell donor, and demonstrated a restricted allospecificity in panel cell studies. One of these, γ/δ TLC RNG‐135, was studied in more detail. Its phenotype was CD3 + TcR α/β − TcR γ/δ + BB3 − δTCS1 + CD4 − CD8 − . Inhibition experiments using monoclonal antibodies indicated that the cytotoxicity of TLC RNG‐135 was mediated through the TcR γ/δ, and directed against an HLA‐DQ molecule. In extended panel cell experiments, this γ/δ TLC only lysed cells carrying the DQA1*0501 and DQB1*0301 genes, either in cis position (on the DR5, DQw7 haplotype), or in trans position (the donor of the stimulating cells, DR3,4; DQw2, w7). Thus, it appears that γ/δ T cells may recognize a particular HLA‐DQ α/β heterodimer, which may be encoded by DQA1 and B1 genes both in cis and trans position.