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Camp inhibits the okt3‐ induced increase in cytoplasmic free calcium in the jurkat t cell line: the degree of inhibition correlates inversely with the amount of cd3 binding ligand used *
Author(s) -
Papadogiannakis Nikos,
Nordström Tommy E.,
Andersson Leif C.,
Wolff C. Henrik J.
Publication year - 1989
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830191029
Subject(s) - jurkat cells , forskolin , intracellular , biology , signal transduction , cell culture , microbiology and biotechnology , t cell , endocrinology , medicine , receptor , biochemistry , immunology , immune system , genetics
Abstract We have investigated the effect of cAMP concentration on the CD3‐mediated rise in intracellular Ca 2+ induced by anti‐CD3 monoclonal antibody in the human T cell leukemia line Jurkat. Forskolin, prostaglandin E 2 (PGE 2 ) and dibutyryl cAMP (db‐cAMP) were used to increase intracellular cAMP concentrations. Treatment of Jurkat cells with forskolin or db‐cAMP for 3 h inhibited the subsequent rise in intracellular Ca 2+ concentration induced by an optimally mitogenic dose of 100 ng/ml of the anti‐CD3 OKT3, whereas PGE 2 counteracted the Ca 2+ rise only marginally. The inhibitory effect of forskolin and PGE 2 on the Ca 2+ signal correlated with their abilities to induce increased cAMP levels in Jurkat cells. The suppression of the Ca 2+ response was dependent on the concentration of the cAMP‐elevating agent and the time of pre‐incubation with the drug. The cAMP‐mediated inhibition of the Ca 2+ response diminished or disappeared when increasing concentrations of OKT3 were used to stimulate the rise in intracellular Ca 2+ concentration. The results indicate that cAMP participates in the regulation of T lymphocyte activation at the level of signal transduction. Our findings, which stress the crucial role of the concentration of the ligand used to trigger Ca 2+ responses, provide an explanation to previous contradictory reports on the impact of cAMP on the signal transduction in T cells.