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Inactivation of rabbit immunoglobulin λ chain variable region genes by the insertion of short interspersed elements of the C family
Author(s) -
Hayzer David J.,
Jaton JeanClaude
Publication year - 1989
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830190919
Subject(s) - pseudogene , biology , gene , genetics , exon , gene cluster , microbiology and biotechnology , gene family , coding region , stop codon , immunoglobulin light chain , antibody , gene expression , genome
Abstract Two rabbit germ‐line genes encoding immunoglobulin λ light chain V regions were cloned from a rabbit genomic liver DNA library and characterized. One, V λ 1, is separated by at least 8 kb from any other V λ gene. The second, V λ 4, forms part of a three‐gene cluster with two functional V λ genes recently reported. Both V λ 1 and V λ 4 have structural features rendering them pseudogenes. The coding regions have frame‐shift mutations which would yield defective protein products; both genes are also interrupted by the insertions of short, interspersed repetitive elements of the C family. In the V λ 1 gene, the 369‐bp insert is located upstream of the gene between the putative TATA box and the leader exon, whereas in gene V λ 4, the 360‐bp insert interrupts the FR2 at codon 48c. In addition, the sequence of the complement‐determining region 3 of gene V λ 1 is very similar to the mouse DSP2.6 sequence.