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cDNA cloning of functional T cell receptor γ/δ chains expressed in human peripheral blood lymphocytes
Author(s) -
MamiChouaib Fathia,
Jitsukawa Setsuko,
Faure Florence,
Vasina Bruno,
Genevee Catherine,
Hercend Thierry,
Triebel FréDéRic
Publication year - 1989
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830190905
Subject(s) - biology , microbiology and biotechnology , cloning (programming) , monoclonal antibody , complementary dna , phenotype , somatic cell , molecular cloning , epitope , gene , genetics , antibody , computer science , programming language
Abstract We have identified in earlier studies two V δ rearrangements corresponding to a 4.5‐kb Eco RI fragment detected with a V δ 1 probe and to a 7‐kb Eco RI band detected with a V δ 2 probe. These rearrangements have been found in two human T cell clones, F6C7 and G6, displaying surface phenotypes unfrequent in human peripheral blood, namely Ti γ A + BB3 ‐ (F6C7) and Ti γ A ‐ BB3 + (G6). Herein, we report the sequences of the functional transcripts encoded by these rearranged genes and show that the 4.5‐and the 7‐kb Eco RI fragments correspond to V1/D3/J δ 3 and to V2/D3/J δ 3 recombinations, respectively. In addition, we have sequenced the V2/D3/J1/C δ transcripts expressed in two clones, AB12 and VTC, which have a Ti γ A + BB3 + surface phenotype corresponding to that of most γ/δ peripheral lymphocytes. Analyses of the δ transcripts expressed by these four cells further strengthen the hypothesis that anti‐BB3 and anti‐δ‐TCS‐1 monoclonal antibodies recognize a V δ 2‐ and a V1/(D)/J δ 1‐encoded epitope, respectively. Sequence of the γ transcripts expressed by AB12 and F6C7 cells shows that they encode a V9/JP/C γ 1 chain. Finally, we confirm that non‐combinatorial diversity in the γ and δ proteins is generated by both junctional flexibility and N‐region addition without any somatic mutation.