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Regulation of immunoglobulin gene expression in trans by phorbol esters
Author(s) -
Mårtensson IngaLill,
Iglesias Antonio,
Leanderson Tomas
Publication year - 1989
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830190823
Subject(s) - biology , microbiology and biotechnology , rna , gene expression , isotype , gene , enhancer , transcription (linguistics) , reporter gene , regulatory sequence , regulation of gene expression , dna , antibody , genetics , monoclonal antibody , linguistics , philosophy
Abstract Lipopolysaccharide‐stimulated B cell cultures treated with phorbol‐12,13‐dibutyrate have been shown to down‐regulate their steady state levels of μs RNA while the μm RNA remained constant. In contrast, the steady state levels of δ RNA was upregulated by the same treatment. By using mice transgenic for a rearranged μ or δ gene, it could be shown that the down‐regulatory effect acts in trans on a defined DNA stretch and is independent of isotype. Thus, the positive effect on endogenous δ RNA steady‐state levels must be regulated at the level of RNA processing. In addition, a DNA construct containing 1.5 kb of a ϵ promoter 5′ of, and an IgH enhancer 3′ of, a chloramphenicol acetyl transferase reporter gene could be down‐regulated in trans by anti‐μ or phorbol‐12,13‐dibutyrate treatment, showing that transcriptional control of Ig promoter elements can readily be observed in non‐transformed B lymphocytes.