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Rearranging sequence located in the intron of the human T cell receptor γ chain gene constant region
Author(s) -
Chen Zhu,
Loiseau Pascale,
Bories Jean Christophe,
Bensussan Armand,
Sigaux FrançOis
Publication year - 1989
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830190410
Subject(s) - biology , intron , t cell receptor , microbiology and biotechnology , gene , exon , genetics , gene rearrangement , clone (java method) , rna splicing , gamma subunit , alternative splicing , t cell , protein subunit , rna , immune system
Abstract Human T cell rearranging γ genes encode membrane proteins which are expressed by a minor subset of T cells. Extensive studies have shown that TcR γ gene rearrangements result from V γ ‐J γ recombinations. Here we analyze an unusual rearrangement involving a non‐translatable sequence flanked by heptamer and nonamer signals in a Ti α/β‐positive T cell clone (JF1). This sequence, designated here as I γ RS, is distinct from V, D or J elements. It is located in the first intron of the first constant region (C γ 1) and homologous sequences are conserved in C γ 2 introns. In JF1 cells the I γ RS is juxtaposed to the J γ 2 segment downstream to a V γ 10‐J γ 1 rearrangement. The use of a cryptic splicing site induced JF1 cells to produce an aberrant large‐sized transcript containing the I γ RS 3′ to the first exon of C γ 1. Such a rearrangement occurring downstram to an inframe V γ ‐J γ junction may induce premature arrest of translation and lack of the C‐terminal part of C γ involved in γ‐protein assembly.