Premium
A MEMBER OF A NEW V H GENE FAMILY ENCODES ANTI‐BROMELINIZED MOUSE RED BLOOD CELL AUTOANTIBODIES
Author(s) -
Reininger Luc,
Kaushik Azad,
Izui Shozo,
Jaton JeanClaude
Publication year - 1988
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830181008
Subject(s) - biology , gene , microbiology and biotechnology , complementary dna , clone (java method) , gene family , genetics , nucleic acid sequence , immunoglobulin heavy chain , gene expression
Abstract A cDNA clone encoding the variable region of the heavy chain of a BALB/c anti‐bromelinized mouse red blood cell (BrMRBC) monoclonal antibody has been characterized. The nucleic acid sequence indicates that the variable region of the heavy chain is likely encoded by variable‐VCP12, diversity‐DSP 2 (5, 7 or 8) and joining‐J H 1 germ‐line genes. An identical combination of V genes was observed for six other CBA/J and NZB anti‐BrMRBC hybridomas. The BALB/c VCP12 nucleotide sequence is less than 80% homologous to members of the 10 known V H families. Moreover, the genomic restriction fragments detected under moderate stringency conditions with the radiolabeled cDNA probe did not correspond to those obtained with probes of the most homologous V H families (7183 and X24). The results indicate that the VCP12 gene defines a new V H family which we propose to designate v H 11. The observation of 1, 2 or 3 enomic restriction fragments at the most, with mice bearing the Igh‐V d or J , Igh‐V a or b F or Igh‐V c haplotypes, suggests the existence of a few VCP12‐related genes.