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Relationship of B cell Fc receptors to T cell recognition of Mls antigen
Author(s) -
Nicolas JeanFrancois,
Wegmann Dale,
Lebrun Philippe,
Kaiserlian Dominique,
Tovey Julie,
Glasebrook Andy L.
Publication year - 1987
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830171106
Subject(s) - biology , antigen , microbiology and biotechnology , t cell , locus (genetics) , receptor , monoclonal antibody , b cell , antibody , t cell receptor , t lymphocyte , immune system , immunology , gene , genetics
Abstract The Mls locus on chromosome 1 controls the expression of cellular determinants that are responsible for stimulating mixed lymphocyte reactions between H‐2‐identical strains. However, the biochemical nature of Mls antigenic determinants remains undefined. It has been proposed that Ly‐17 lymphoid cell surface antigens (also known as Ly.m.20.2 and LyM‐1) and Mls antigens could be identical because they are both encoded by loci on chromosome 1 and display similar tissue distribution. The Ly‐17 locus encodes polymorphic alleles of the IgG Fc receptor (FcγR). In the present study, two approaches were used to address the question of whether FcγR are involved in T cell recognition of Mls antigen. In the first approach we tested the effect of FcγR blockade by heat‐aggregated mouse IgG or anti‐FcγR monoclonal antibodies (2.4.G2) on the ability of an Ia + , FcγR + Mls a ‐expressing B cell hybrid (LBB.3.4.16) to stimulate interleukin 2 secretion by an anti‐Mls a ‐specific T cell hybrid. We show that blockade of FcγR does not inhibit the Mls a ‐specific stimulation of T cells during a 24‐h culture period in which FcγR remain blocked. In the second approach, we derived irradiation‐induced variants of LBB.3.4.16 to dissociate FcγR expression and Mls antigen expression. We describe 2 LBB variants which no longer stimulate Mls a ‐reactive T cells but do express FcγR. Compared to parental LBB cells, the capacity of variant LBB cells to present soluble antigen to Ia‐restricted T cells is unaffected. Collectively, these results indicate that FcγR expression and Mls a antigen stimulation can be dissociated. We conclude that FcγR expression may be necessary, but not sufficient for T cell recognition of Mls antigen.