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Discrimination of hepatocyte‐stimulating activity from human recombinant tumor necrosis factor α
Author(s) -
Andus Tilo,
Heinrich Peter C.,
Bauer Joachim,
TranThi ThuyAnh,
Decker Karl,
Männel Daniela,
Northoff Hinnak
Publication year - 1987
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830170817
Subject(s) - tumor necrosis factor alpha , acute phase protein , hepatocyte , biology , recombinant dna , in vivo , macroglobulin , albumin , immunology , necrosis , medicine , endocrinology , microbiology and biotechnology , in vitro , biochemistry , inflammation , gene , genetics
Abstract The involvement of tumor necrosis factor α (TNFα) in the regulation of acute‐phase protein synthesis is currently under discussion. In this study the effect of human recombinant TNFα on the regulation of the 4 acute‐phase proteins α 2 ‐macroglobulin, albumin, α 1 ‐proteinase inhibitor and α‐acute‐phase globulin was investigated in rat hepatocyte primary cultures. No changes in synthesis of any of the 4 proteins were observed. However, an acute‐phase response similar to that in vivo could be generated by conditioned media from human monocytes containing natural TNFα. This response remained unchanged after neutralizing TNFα activity by the addition of a specific antibody to TNFα. It is concluded that the hepatocyte‐stimulating activity synthesized by human monocytes is different from TNFα.