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The induction of helper and suppressor cells with secondary anti‐hen egg‐white lysozyme B hybridoma cells in the absence of antigen
Author(s) -
Kawahara Douglas J.,
Miller Alexander,
Sercarz Eli E.
Publication year - 1987
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830170805
Subject(s) - biology , monoclonal antibody , population , antigen , idiotype , microbiology and biotechnology , in vitro , immunology , antibody , b cell , genetics , medicine , environmental health
Abstract The results presented in this report define a dominant T cell‐recognized public idiotype (SRId) expressed on monoclonal anti‐chicken egg‐white lysozyme (HEL) antibodies produced by hybridomas derived from secondary response lymphocytes. This Id mediates interactions between SRId + B cells and SRId‐recognizing T cells. In the absence of exogenous antigen, irradiated secondary anti‐HEL B hybridoma cells (B‐Hyb) of nonoverlapping specificity can be used to induce a helper T cell population capable of specifically stimulating an in vitro anti‐HEL plaque‐forming cell (PFC) response. Importantly, similar immunizations using carbodiimide‐treated secondary anti‐HEL B‐Hyb cross‐primed for a suppressor T cell population capable of suppressing this in vitro anti‐HEL PFC response. That is, suppression was seen not only to the response induced by the homologous B‐Hyb but to other B‐Hyb which express anti‐HEL monoclonal antibody of nonoverlapping specificity. This evidence is consistent with the presence of a pre‐existent regulatory Id network involving SRId in antigennaive animals. After immunization with HEL, regulatory cells exert a strong selective pressure which leads to a secondary anti‐HEL B population, of varying fine specificity, but uniformly positive for SRId.