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Shared idiotopes between anti‐class II monoclonal antibodies and major histocompatibility complex recognition site of T cells
Author(s) -
Tsuji Moriya,
Yagi Junji,
Abe Ryo,
Asano Yoshihiro,
Tada Tomio
Publication year - 1987
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830170515
Subject(s) - biology , idiotopes , monoclonal antibody , major histocompatibility complex , allorecognition , microbiology and biotechnology , antibody , antigen , t cell , t lymphocyte , mhc restriction , immunology , mhc class i , monoclonal , immune system
Abstract We have raised a number of monoclonal antibodies (mAb) against idiotopes (Id) on monoclonal anti‐murine class II (anti‐A k or E k ) antibodies. Two anti‐Id mAb among 31 were found to cross‐react with some T cells but not with macrophages and B cells of H‐2 k animals. They were able to block syngeneic mixed lymphocyte reaction (SMLR) and antigen‐induced major histocompatibility complex (MHC)‐restricted T cell proliferation of H‐2 k but not of other haplotypes. These results indicated that antibodies were recognizing Id associated with the MHC restriction site of T cells. The injection of these anti‐Id mAb into H‐2 k mice resulted in the stimulation of self‐class II‐reactive T cell clones as determined by SMLR. They were also able to stimulate in non‐H‐2 k strains to prime H‐2 k alloreactive T cell clones. Some animals developed anti‐class II (anti‐Ia k ) antibodies by the injection of anti‐Id. These results indicated that certain anti‐Id (anti‐anti‐class II) antibodies cross‐reacted with T cell receptors which carried class II restriction specificity and were involved in allorecognition. These antibodies were found to have an ability to alter the T cell repertoire in vivo by stimulating such MHC‐restricted clones.