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Targeting cytotoxic T cells to antigen‐specific B lymphocytes
Author(s) -
Rammensee HansGeorg,
Julius Michael H.,
Nemazee David,
Langhorne Jean,
Lamers Rinus,
Köhler Georges
Publication year - 1987
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830170322
Subject(s) - cytotoxic t cell , biology , t cell receptor , antigen , antibody , ctl* , microbiology and biotechnology , receptor , antigen presenting cell , t cell , immunology , immune system , in vitro , cd8 , biochemistry
Abstract A recent development in immunomanipulation involves the targeting of cytotoxic T lymphocytes (CTL) to cell‐bound antigens using bispecific antibodies. These antibodies have been engineered such that specificity is directed against the T cell receptor (TCR) or TCR‐associated T3 molecules, as well as against the chosen antigen. The present study was aimed to force interactions between T and B cells by bridging their receptors. F23.1 antibodies, which are specific for gene products of the TCR V β 8 gene family, were conjugated with TNP (2,4,6‐trinitrophenyl) and this construct was used to bridge the receptors of V β 8 + T cells with the receptors of TNP‐specific B cells. The bridging was demonstrated by direct killing of both a TNP‐specific B hybridoma and of blast cells from mice transgenic for μ, x of the TNP‐specific antibody Sp6. Further, F23.1‐TNP constructs in conjunction with V β 8 + CTL were shown to specifically deplete Ig‐secreting B cells from Sp6 transgenic mice. Conjugates of TCR‐specific antibodies and antigen are theoretically useful in vivo to either deplete or expand B cells of a given specificity by coupling their receptors to the TCR of CTL or T helper cells, respectively.