Premium
A surface molecule on guinea pig lymphocytes involved in adhesion and homing
Author(s) -
Kraal Georg,
Twisk Agaath,
Tan Ben,
Scheper Rik
Publication year - 1986
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830161208
Subject(s) - homing (biology) , biology , high endothelial venules , spleen , cell adhesion molecule , lymph node , population , lymphocyte homing receptor , in vitro , guinea pig , immunology , antigen , microbiology and biotechnology , lymph , monoclonal antibody , antibody , cell adhesion , cell , pathology , endocrinology , biochemistry , medicine , ecology , demography , sociology
Abstract A monoclonal antibody, CT4, which recognizes an antigenic determinant on the majority of guinea pig lymphocytes, was tested for its ability to interfere with adherence and homing capacity of lymphocytes. Incubation with F(ab′) 2 fragments of the antibody blocks the in vitro binding to high endothelial venules (HEV) of both peripheral lymph nodes and Peyer's patches. When tested in vivo using a short‐term homing assay with radiolabeled cells also a reduction of migration into the spleen was observed. Fluorescence‐activated cell sorter analysis of lymph node cells showed a separation into duller and brightly positive cells whereas in the thymus the bright population is absent. Thymus cells adhere less effectively to HEV and this binding can only marginally be blocked by CT4 incubation. The results suggest a role of CT4 in adhesion processes.