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A protective human monoclonal IgA antibody produced in vitro : Anti‐pneumococcal antibody engendered by Epstein‐Barr virus‐immortalized cell line
Author(s) -
Steinitz Michael,
Tamir Sara,
Ferne Mina,
Goldfarb Ada
Publication year - 1986
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830160214
Subject(s) - antibody opsonization , biology , antibody , monoclonal antibody , microbiology and biotechnology , streptococcus pneumoniae , virology , in vitro , clone (java method) , virus , cell culture , immunology , dna , opsonin , biochemistry , genetics , antibiotics
Abstract Human lymphocytes that produce anti‐pneumococcal antibodies were separated and immortalized by Epstein‐Barr virus and then cloned. One clone (NAD‐Sel) produces an IgA, x antibody which is specific for the polysaccharides of type 8 pneumococcus, while not reactive with any of the polysaccharides derived from 24 other pneumococcal strains. The antibody, which is present in the cell supernatant as monomer and polymer, binds to protein A and does not fix complement. When incubated in vitro with type 8 pneumococci, it induces direct killing and increases the opsonization of these bacteria by mouse macrophages.