Premium
Eosinophil activation by lymphokines and T cell clone products in the rat
Author(s) -
Veith Monica,
Pestel Jöel,
Loiseau Sylvie,
Capron Monique,
Capron André
Publication year - 1985
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830151218
Subject(s) - biology , lymphokine , eosinophil , concanavalin a , immunology , immunoglobulin e , schistosoma mansoni , clone (java method) , eosinophil peroxidase , antigen , microbiology and biotechnology , t cell , antibody , in vitro , immune system , schistosomiasis , biochemistry , helminths , dna , asthma
Abstract T cell lines and T cell clones derived from inbred Fischer rats and specific for Schistosoma mansoni antigens were established. Cell‐free supernatants from the T cell lines demonstrated a marked capacity to enhance IgE‐ and IgGa‐dependent eosinophil mediated killing of S. mansoni larvae in vitro . In addition, supernatants from cloned T cells stimulated with concanavalin A or specific antigen, or unstimulated, enhanced IgE‐dependent eosinophil‐mediated helminthotoxicity. The enhancing activity in both cases was very heat‐stable (100°C, 10 min). We also found that clone‐derived supernatants enhance eosinophil peroxidase release upon stimulation with homologous IgE and anti‐IgE as well as inducing a more delayed spontaneous release of peroxidase. In view of the established thymus dependency for the development of immunity to schistosomiasis in the rat, the availability of these s. mansoni ‐specific cloned T cells has enabled the relationship between eosinophils, lymphocytes and anaphylactic antibodies to be examined more closely.