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A neomycin‐resistant cell line for improved production of monoclonal antibodies to cell surface antigens
Author(s) -
van Snick Jacques,
de Plaen Etienne,
Boon Thierry
Publication year - 1985
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830151116
Subject(s) - neomycin , biology , monoclonal antibody , spleen , microbiology and biotechnology , antigen , transfection , cell culture , cell fusion , plasmid , antibody , cell , immunology , gene , antibiotics , genetics
Abstract Overgrowth of hybridomas by proliferating spleen T cells often hinders the production of monoclonal antibodies to certain antigens. To overcome this problem we derived neomycin‐resistant fusion partner SP2 neo R .l by transfection of SP2/0‐Agl4 cells with plasmid pSVTK neo beta. Hybridomas obtained with SP2 neo R .1 grew optimally in the presence of the neomycin analog G418 at concentrations which blocked the proliferative response of T cells to mitogenic stimuli. The advantage of using SP2 neo R .1 and G418 under conditions where spleen cell proliferation occurs after fusion was demonstrated with hybridomas derived from a rat immunized with mouse helper T cell lines.