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Induction of cytolytic cells by pure recombinant human interleukin 2
Author(s) -
Devos Rene,
Plaetinck Geert,
Fiers Walter
Publication year - 1984
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830141118
Subject(s) - biology , cytolysis , concanavalin a , chinese hamster ovary cell , recombinant dna , cytotoxic t cell , interleukin 2 , lymphokine , microbiology and biotechnology , lymphocyte , cell culture , t lymphocyte , immunology , cytokine , immune system , in vitro , biochemistry , genetics , gene
Abstract Purified recombinant human interleukin 2, produced in Escherichia coli , was sufficient to generate cytolytic cells in concanavalin A‐stimulated, T helper cell‐depleted (Lyt‐1.1 − ) or accessory cell‐depleted (Ia − ) murine spleen cell cultures. Moreover, recombinant interleukin 2 (rIL 2) was able (even at 0.5 ng/ml) to induce cytolytic cells in undepleted murine spleen cell cultures and undepleted human peripheral blood lymphocyte cultures in the absence of a mitogen. Purified recombinant human interferon‐γ, produced in Chinese hamster ovary cells, did not induce either cytolytic activity or IL2 responsiveness in human peripheral blood lymphocyte cultures. Possibly, the rIL2‐induced cytolytic cells are formed as a result of nonspecific secondary cytotoxic T lymphocyte activation.