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Frequency analysis of cytolytic T lymphocyte precursors (CTL‐P) generated in vivo during lethal rabies infection of mice. II. Rabies virus genus specificity of CTL‐P
Author(s) -
Reddehase Matthias J.,
Cox James H.,
Koszinowski Ulrich H.
Publication year - 1984
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830141114
Subject(s) - ctl* , biology , virology , rhabdoviridae , lyssavirus , rabies , rabies virus , virus , vesicular stomatitis virus , cytolysis , mononegavirales , immunology , antigen , cytotoxic t cell , in vitro , viral disease , paramyxoviridae , cd8 , biochemistry
Abstract Cytolytic T lymphocyte precursors (CTL‐P) were sensitized in vivo by intraplantar infection of C57BL/6 mice with a lethal dose of rabies virus, strain ERA (ERA). As a result of sensitization CTL‐P matured to interleukin‐receptive CTL‐P (IL‐CTL‐P) that could be expanded in vitro to Thy‐1 + , Lyt‐2 + CTL clones in the presence of IL without subjection to antigen‐driven selection. After infection with ERA, IL‐CTL‐P‐derived CTL lysed fibroblasts infected with rabies virus but not those infected with another rhabdovirus, the vesicular stomatitis virus. These CTL, however, did not discriminate between fibroblasts infected with the serologically closely related laboratory strains of classic rabies virus, ERA and HEP‐Flury, and the serologically distinct rabies‐related African isolate Mokola. This finding implies that in vivo sensitized IL‐CTL‐P recognize common genus‐specific determinants expressed on cells infected with members of the lyssavirus genus.