Premium
Absence of cell surface fixation of a monoclonal antibody detectable by conventional immunoassays does not exclude expression of and interaction with the corresponding antigenic determinant
Author(s) -
Layet Corine,
Bouteiller Philippe P. Le,
Olive Daniel,
Mishal Zohair,
Caillol Danielle,
Kourilsky François M.,
Jordan Bertrand R.,
Lemonnier François A.
Publication year - 1984
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830140118
Subject(s) - monoclonal antibody , radioimmunoassay , biology , microbiology and biotechnology , antigen , human leukocyte antigen , antibody , cell , epitope , monoclonal , immunology , biochemistry
Abstract No specific binding of anti‐HLA class I B.10.6 monoclonal antibody (mAb) could be demonstrated by cell surface radioimmunoassay and cytofluorographic studies at the surface of murine transformed L cells expressing HLA‐A3 or Cw3 molecules. However, specific interaction of this antibody with these molecules at the surface of these transformed cells was indirectly established, since it inhibited specifically the binding to the same HLA class I molecules of other anti‐HLA class I mAb. Therefore, the absence of detectable binding of mAb, in conventional immunoassays, does not exclude expression by these cells of the corresponding antigenic determinant.