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Selective inhibition of antigen‐induced “step one” in cytotoxic T lymphocytes by anti‐Lyt‐2 antibodies
Author(s) -
Gullberg Martin,
Larsson EvaLotta
Publication year - 1982
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830121205
Subject(s) - ctl* , cytotoxic t cell , antigen , biology , immunology , t lymphocyte , interleukin 2 , monoclonal antibody , antibody , cytolysis , lymphokine , cytotoxicity , microbiology and biotechnology , immune system , in vitro , biochemistry , cd8
Abstract Stimulation of mixed lymphocyte cultures with ultraviolet (UV)‐irradiated stimulator cells leads to selective activation of Lyt−2 + cytotoxic T lymphocyte precursurs (CTL‐P) to acquire interleukin 2 (IL2) reactivity: (a) no proliferative response, nor IL2 production was observed in these cultures; (b) upon addition of preformed Unconditioned media (IL2‐CM), a vigorous proliferative response was observed; (c) the IL2‐dependent proliferation was reduced by approximately 90% after depletion of Lyt−2 + responder cells; and (d) specific CTL were generated upon the initial triggering by UV‐irradiated stimulators and IL2 expansion. This system therefore provides suitable conditions for studying the role of Lyt‐2 antigens in initial, antigen‐specific induction of CTL‐P, as well as at their effector phase. It is shown herein that monoclonal anti‐Lyt‐2 antibodies inhibit antigen‐specific acquisition of IL2 reactivity by CTL‐P, which results in 85% reduction of the proliferative response mediated by IL2‐CM. CTL‐P which have been induced by antigen to IL 2 reactivity, prior to addition of anti‐Lyt‐2 antibodies, however, are not affected in their IL2‐dependent proliferation. Furthermore, both the specific and lectindependent cytolytic activity of effector CTL generated by UV‐irradiated stimulators and IL2‐CM is blocked by addition of anti‐Lyt‐2 antibodies in the cytotoxicity assay. The implications of these findings for the role of Lyt‐2 antigen in antigen recognition by T cells are discussed.