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Use of monoclonal anti‐idiotypic antibody to P3‐X63Ag8 myeloma protein for analysis and purification of B lymphocyte hybridoma products
Author(s) -
Gheuens Jan,
McFarlin Dale E.
Publication year - 1982
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830120815
Subject(s) - monoclonal antibody , biology , myeloma protein , radioimmunoassay , antibody , microbiology and biotechnology , lymphocyte , hybridoma technology , multiple myeloma , monoclonal , immunology , virology , biochemistry
Abstract A lymphocyte hybridoma, termed 80 V5B4, that secretes a syngeneic anti‐idiotypic antibody to P3‐×63Ag8 (×63) myeloma protein, is described. A sensitive radioimmunoassay to detect ×63 myeloma protein was developed with this monoclonal anti‐idiotypic antibody which can also be used to remove the ×63 myeloma protein from hybridoma products.