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Selective suppression of the cytotoxic T cell response to influenza virus in mice
Author(s) -
Leung KwokNam,
Ashman Robert B.,
Ertl Hildegund C. J.,
Ada Gordon L.
Publication year - 1980
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830101102
Subject(s) - cytotoxic t cell , adoptive cell transfer , virus , biology , sendai virus , antibody , virology , spleen , immune system , t cell , microbiology and biotechnology , immunology , in vitro , biochemistry
Abstract Mice injected with inactivated (UV light‐irradiated) influenza virus produce specific antibody, become sensitized for a delayed‐type hypersensitivity reaction, but do not generate specific cytotoxic T (T c ) cells. If injected 4–5 days later with infectious virus, the formation of T c cells is suppressed by > 90%. If A strain viruses are used, the suppression observed is cross‐reactive within A strain viruses but does not extend to B/LEE or to Sendai virus. Serum from mice injected with UV‐irradiated virus contains antibodies which on adoptive transfer can inhibit T c cell formation when infectious homologous virus is used to challenge the recipients. Spleen cells from the same mice, upon adoptive transfer, also inhibit (50–70%) T c cell formation if transferred within 24 h of injection of infectious virus, and the specificity pattern observed is cross‐reactive within A strains. The activitiy of the cells mediating suppression is destroyed by monospecific anti‐Thy‐1.2 antibody and complement. The immune cells require I region sharing between donor and recipient mice for their suppressor activity to be effective. (There is also a partial requirement for K. D region sharing, but the possible rejection of transferred cells is not excluded.) Dilution assays in which clonal expansion of T c precursors is used to estimate their frequency and the presence of T helper (T h ) cells indicate that suppressed mice possess T c precursors and primed cells which, upon restimulation, act as T h cells. Furthermore, injection of irradiated T h cells with inactivated virus does not significantly reduce the ensuing suppression.