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Idiotypic specificities which arise naturally in rabbits following allotype suppression of the major V H subgroup
Author(s) -
Roux Kenneth H.,
GilmanSachs Alice,
Dray Sheldon
Publication year - 1980
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830100708
Subject(s) - allotype , idiotype , biology , locus (genetics) , microbiology and biotechnology , antibody , immunology , genetics , gene , monoclonal antibody
Abstract In contrast to normal IgG, some of the IgG molecules, which appear in compensation as a result of allotype suppression of IgG molecules of the major V H subgroup (a locus), were found to have an easily identifiable idiotypic specificity. For each of three donors' IgG, a different idiotypic specificity was present, and each idiotypic specificity was found on the Fab, as expected, but not on the Fc fragment. Further analysis of the IgG of one donor revealed that the idiotypic specificity was present on 15% of the Fab γ fragments. The idiotypic specificity was expressed on IgG which also bore an allotypic specificity of the y locus V H subgroup of IgG which comprised most (72%) of the total compensatory IgG. This suggests that suppression of clones synthesizing the a locus major V H subgroup results in compensation by minor V H subgroup clones more limited in variability. These clones may then proliferate to synthesize larger amounts of IgG bearing the same idiotypic specificity which thereby becomes more easily detectable by immunization of rabbits.