Premium
Production of interferon in the murine mixed lymphocyte culture. II. Interferon production is a T cell‐dependent function, independent of proliferation
Author(s) -
Kirchner Holger,
Zawatzky Rainer,
Engler Helmut,
Schirrmacher Volker,
Becker Hans,
von Wussow Peter
Publication year - 1979
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830091015
Subject(s) - biology , interferon , puromycin , spleen , immunology , mixed lymphocyte reaction , lymphocyte , microbiology and biotechnology , virology , t cell , immune system , protein biosynthesis
Abstract Interferon production occurs after two days of culture in murine mixed lymphocyte cultures (MLC). This was demonstrated in various combinations of mouse spleen cells differing at the major histocompatibility (H‐2) locus. Interferon production could be demonstrated in one‐way MLC when F 1 /parent combinations were used and in reactions in which one partner was treated by puromycin. After treatment of both cell populations with mitomycin C, interferon production occurred in the absence of lymphoproliferation. Interferon production in response to alloantigen did not occur in spleen cell cultures of nude mice and in cultures treated by anti‐Θ antiserum plus complement indicating that interferon production is a T cell‐dependent function.