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Expression of Ly‐6 alloantigen during differentiation of cytotoxic T cells
Author(s) -
Horton Michael A.,
Beverley Peter C. L.,
Simpson Elizabeth
Publication year - 1979
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830090502
Subject(s) - cytotoxic t cell , antiserum , biology , antigen , in vitro , microbiology and biotechnology , immunology , biochemistry
Abstract We have prepared anti‐Ly‐6.2 by immunization of (CBA/Ca × A‐Thy‐l a )F 1 with AKR/Crc lymphocytes and used the antiserum to study heterogeneity of peripheral T lymphocytes. The Ly‐6 alloantigen is found on all activated cytotoxic T cells studied, directed to a variety of different target antigens. The antiserum reveals heterogeneity among the precursors of T killer cells, i.e. allogeneic precursors are Ly‐6 − , whereas the generation of cytotoxic cells to hapten‐modified syngeneic cells and xenogeneic cells is partially abrogated by serum pretreatment. Precursors of some in vivo primed responses are also Ly‐6 + . These findings indicate that the expression of Ly‐6 antigen is, at least, partially related to the stage of T cell activation. The in vitro proliferative response of T cells to allogeneic Ia antigens is substantially reduced by pretreatment with anti‐Ly‐6.2 serum suggesting that this antiserum may be a useful tool for the study of heterogeneity in the Ly‐l + pool of T cells.