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Induction and characterization of isogeneic anti‐idiotypic antibodies to BALB/c myeloma S117: lack of reactivity with major idiotypic determinants
Author(s) -
Seppälä Ilkka J. T.,
Eichmann Klaus
Publication year - 1979
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830090314
Subject(s) - antibody , idiotype , biology , immunization , immunoglobulin idiotypes , antigen , idiotopes , myeloma protein , microbiology and biotechnology , isoelectric focusing , immunology , monoclonal antibody , biochemistry , enzyme
Abstract Isogeneic anti‐idiotypic antibodies were induced by immunization of BALB/c mice with the BALB/c‐derived myeloma protein S117 which binds N‐acetylglucosamine‐containing antigens including Group A streptococcal carbohydrate (A‐CHO). Most BALB/c mice produced anti‐S 117 idiotypic antibodies, as shown by various different immunization protocols. The antibodies of individual mice were of intermediate to high affinity (2.8 × 10 6 M −1 to 1.4 × 10 8 M −1 ). In isoelectric focusing, most individual antibodies were shown to consist of a small number of clonotypes, but each mouse produced its own unique set of clones so that the potential clonal repertoire of strain BALB/c is rather large. Most importantly, all isogeneic anti‐idiotypic antibodies were directed against idiotypic determinants of S 117 that are not shared with induced anti‐A‐CHO antibodies, whereas it has been shown previously that allogeneic and xeno‐geneic anti‐S 117 idiotypic antibodies react with idiotypic determinants unique to S 117 as well as with those that are shared with anti‐A‐CHO antibodies. The data suggest that the expression of S 117 idiotypic determinants in strain BALB/c is not under antibody‐mediated, anti‐idiotypic feedback control.