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Idiotype suppression. III. Induction of unresponsiveness to sensitization with anti‐idiotypic antibody: identification of the cell types tolerized in high zone and in low zone, suppressor cellmediated, idiotype suppression
Author(s) -
Hetzelberger Doris,
Eichmann K.
Publication year - 1978
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830081204
Subject(s) - idiotype , immunology , sensitization , biology , antibody , t cell , suppressor , b cell , microbiology and biotechnology , immune system , monoclonal antibody , gene , biochemistry
Abstract Unresponsiveness of strain A/J mice to sensitization with guinea pig IgG anti‐A5A idiotypic antibody is induced by prior injection of guinea pig IgG 2 anti‐A5A idiotypic antibody or unfractionated anti‐A5A antiserum. Two different forms of unresponsiveness could be induced depending on the dose of IgG 2 anti‐A5A: high‐zone suppression was maximal one week after injection, could not be transferred by lymphocytes and disappeared within 3 to 4 weeks. Low‐zone suppression took 7 weeks to fully develop and could be transferred by splenic and lymph node T cells. High‐zone suppression is expressed as transient unresponsiveness in both the A5A‐idiotype‐positive T helper as well as B precursor cell compartments. Low‐zone, suppressor cell‐mediated suppression is expressed as chronic unresponsiveness in the A5A‐idiotype‐positive T helper cell compartment only, whereas the A5A‐idiotype‐positive B precursor cells remain fully responsive as revealed by addition of nonsuppressed T helper cells. These data satisfactorily explain that suppression of T helper cell‐independent idiotypes does not involve T suppressor cells. However, since the presence of suppressor T cells is accompanied by selective unresponsiveness of A5A‐positive B cells, the data imply that idiotypic restrictions exist for T‐B cooperation.