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Antibodies to ganglioside G M1 induce mitogenic stimulation and cap formation in rat thymocytes
Author(s) -
Sela B.A.,
Raz A.,
Geiger B.
Publication year - 1978
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830080410
Subject(s) - cytochalasin b , biology , ganglioside , microbiology and biotechnology , stimulation , sodium azide , colchicine , antibody , cytochalasin d , organelle , intracellular , thymocyte , cytoskeleton , biochemistry , cell , t cell , immunology , endocrinology , immune system , genetics
Abstract Antibodies towards the ganglioside G M1 [galactosyl‐N‐acetylgalactosaminyl‐(N‐acetylneuraminyl)‐galactosylglucosyl ceramide] stimulated DNA synthesis in rat thymocytes. No mitogenic stimulation was observed with the monomeric Fab fragment of anti‐G M1 , suggesting that cross‐linking of the gangliosides or associated components was required for activation by these antibodies. Incubation of thymocytes with anti‐G M1 and fluorescein‐labeled anti‐rabbit IgG at 0°C resulted in uniform ring‐like or patchy staining that developed into a pronounced cap upon elevation of temperature. The cap had a characteristic uropod form, enriched with intracellular organelles. Sodium azide and cytochalasin B completely inhibited cap formation, while colchicine was without effect. These results imply a possible direct or indirect association between surface gangliosides and submembraneous cytoskeletal assemblies that control modulation of these surface components and may transmit stimuli to the interior of the cell.