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Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction
Author(s) -
Köhler G.,
Lefkovits I.,
Elliott B.,
Coutinho A.
Publication year - 1977
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830071103
Subject(s) - thymoma , biology , sendai virus , spleen , microbiology and biotechnology , cell fusion , cell culture , lysis , hybrid , immunology , virology , virus , genetics , botany
Abstract We have established 16 independent hybrid lines derived from a fusion between an AKR thyrnoma and mixed leukocyte reaction‐stimulated C57BL/6 spleen cells. The hybrids express both parental H‐2 alleles and show hybrid glucose‐6‐phosphate isomerase activity. Two of the lines showed Fc receptor activity not expressed in the parental thymoma. The AKR Thy‐1.1 allele was expressed in all hybrid lines, but no Thy‐1.2 could be detected. No killing activity was retained in the hybrid cells, probably because Sendai virus‐mediated fusion of killer cells resulted in the lysis of the thymoma fusion partner.