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Demonstration that IgG memory is carried by IgG‐bearing cells
Author(s) -
Okumura K.,
Julius M. H.,
Tsu Theta,
Herzenberg Leonore A.,
Herzenberg L. A.
Publication year - 1976
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830060704
Subject(s) - antibody , allotype , biology , immunoglobulin g , b 1 cell , immunology , immune system , microbiology and biotechnology , surface immunoglobulin , b cell , antigen presenting cell , t cell
Abstract Memory B cells which give rise to JgG antibody‐producing cells were generally assumed to be IgG‐bearing cells. However, recent studies indicating that very few IgG‐bearing cells exist in lymphoid tissue brought this assumption into question. In this study, we examined directly the question whether IgG‐bearing cells contain functional precursors of IgG antibody‐producing cells. Using the adoptive secondary immune response, we demonstrated that Ig‐1b‐bearing cells, isolated with the fluorescence‐activated cell sorter (FACS), are the functional precursors of Ig‐1b‐producing cells. Further, we have enriched IgG 2 and IgG 1 memory B cells using the FACS. The results show that IgG 2 ‐bearing cells are the functional precursors of the IgG 2 antibody‐producing cells. Likewise, the IgG 1 ‐bearing cells are the functional precursors of IgG 1 antibody‐producing cells. Thus, IgG memory cells have surface IgG which indicates the class and allotype commitment of the memory cell and its progeny antibody‐forming cells.