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p‐Azobenzenearsonate‐L‐tyrosine‐mediated helper function in immune responses of guinea pigs and rats
Author(s) -
Becker M. J.,
Ray A.,
Andersson L. C.,
Mäkelä O.
Publication year - 1975
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830050409
Subject(s) - nip , biology , priming (agriculture) , immune system , tyrosine , antigen , guinea pig , dinitrophenyl , endocrinology , thymectomy , medicine , biochemistry , antibody , microbiology and biotechnology , immunology , myasthenia gravis , botany , germination , computer science , programming language
Abstract A small bifunctional antigen (4‐hydroxy‐5‐iodo‐3‐nitrophenyl)acetyl‐ϵ‐aminocaproyl‐L‐tyrosine‐azobenzene‐ p‐arsonate [NIP‐cap‐TYR(ABA)] was found to induce fair humoral antibody formation against NIP‐cap but very little anti‐ABA‐TYR. This was observed in rats and guinea pigs. Prior immunization with ABA‐TYR, either as such or coupled to dodecanoylated bovine serum albumin (lipid‐BSA), primed rats for an enhanced anti‐NIP response to NIP‐cap‐TYR(ABA). An attempt to encourage rats to produce anti‐ABA‐TYR in response to the bifunctional antigen by priming them with NIP‐cap‐lipid‐BSA failed. Priming with ABA‐TYR was dose‐depedent. An injection of 1.5–15 nanomoles per rat primed for an increased production of anti‐NIP while 150 nanomoles did not. Adult thymectomized x‐irradiated rats had a poor anti‐NIP response to the bifunctional antigen if they were reconstituted with T‐enriched lymphoid cells from control mice, but a good response if reconstituted with similar cells from ABA‐TYR‐primed syngeneic rats.