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Early detection of potentially lethal events in T cell‐mediated cytolysis
Author(s) -
MacDonald H. R.
Publication year - 1975
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830050406
Subject(s) - ctl* , cytolysis , biology , lytic cycle , lysis , microbiology and biotechnology , cell , immunology , cytotoxic t cell , virology , biochemistry , in vitro , virus
Abstract The short‐term kinetics of the interaction between mouse cytolytic T lymphocytes (CTL) and 51 Cr‐labeled target cells was investigated. It was found that addition of EDTA to mixtures of CTL and target cells instantaneously blocked de novo lytic interactions, but did not inhibit the release of 51 Cr from already damaged target cells. Using this information, a modified cytolytic assay was developed. By applying this assay to highly active CTL populations generated in secondary mixed leukocyte cultures, it was possible to detect appreciable target cell damage as early as 30 seconds after exposure to CTL. Quantitative studies demonstrated linear relationships between cytolysis and time and between rate of cytolysis and cell number under these assay conditions.