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The participation of thymus‐derived and of bone marrow‐derived lymphocytes of sensitized mice, in the proliferative response to specific antigen, in vitro
Author(s) -
Mugraby Lea,
Gery I.,
Sulitzeanu D.
Publication year - 1974
Publication title -
european journal of immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.272
H-Index - 201
eISSN - 1521-4141
pISSN - 0014-2980
DOI - 10.1002/eji.1830040603
Subject(s) - biology , spleen , bone marrow , in vitro , antigen , immunology , microbiology and biotechnology , b 1 cell , antibody , t cell , antigen presenting cell , immune system , biochemistry
Abstract Spleen cells of mice primed with sheep red cells (SRC) responded with increased thymidine uptake when stimulated with SRC in vitro . Depletion of bone marrow‐derived (B) cells by filtering the spleen cells through columns of plastic beads coated with anti‐mouse immunoglobulin serum resulted in a drastic loss of the proliferative response. A similar loss occurred on depleting the thymus‐derived (T) cells by treatment with anti‐Θ serum and complement. Addition of peritoneal exudate cells failed to restore the reactivity of the purified T cells to SRC. The proliferative response could be restored to a level exceeding the sum of the individual responses, by mixing the purified T with the purified B cells. A synergistic interaction between T and B cells is suggested.